1.
2. Our new bacteria have ampicillin resistance and they glow under ultraviolet light.
3. Since we have around 200 bacteria in our amp/ara/LB plate, and 150 in our amp/ara plate, and all of these took in the GFP gene, there must be at least 350 bacteria that took in the GFP gene. If the absorption rate is around 1 in 3, then there were probably around 1000 bacteria in the 100 microliter solution.
4. The purpose of arabinose is to activate the GFP gene and serve as a method of controlling the glow. If the arabinose is not present, bacteria will not produce GFP even if they have the gene.
5. GFP can be used to track the spread of bacteria with a certain trait. They are also an easy way to indicate whether an organism is genetically modified or not. GFP can be used to track the movements of certain cancers like osteosarcoma in dogs.
6. Another use of genetic engineering is to increase crop yield without spending large periods of time on breeding the perfect plant. Genetic engineering may be extremely important in the future with humanity's rising population and need for quick access to food.
The amp/ara/LB plate with bacteria fluorescing under UV light
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